Device

Part:BBa_K1329003:Design

Designed by: Daniela Geist   Group: iGEM14_Marburg   (2014-10-07)


Hag-D2-Strep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 588


Design Notes

GSGSGS-Linkers as flanks of the Strep-Tag for better exposure to the outside


Source

Genome of Bacillus Subtilis Genome of Salmonella Typhimurium Protein sequence of the Strep-Tag was found via pdb.org

References